On β-Galactosidase activity

β-galactosidase in larynx cancer

es, such as α-mannosidases (MAN) and β-galactosidases (GAL), are found in different glycoside hydrolase sequencebased families. Considerable research has proved plays the role of MAN, which play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer. Therefore the study aim was to investigate the activities of MAN and GAL in larynx...

The Influence of Microencapsulation with a Modified Chitosan (Water Soluble) on β- Galactosidase Activity

Effects of Different Fixatives on -Galactosidase Activity

Galactosidase ( -Gal) staining is widely used to demonstrate specific gene expression during evaluation of gene targets in vivo. This technique is extremely sensitive to fixation. Optimal fixation conditions are necessary to obtain the maximal -Gal activity. In this experiment, Carnoy’s and three different aldehyde fixatives were used at different temperatures and over different time points. Ki...

Ratiometric electrochemical detection of β-galactosidase.

A novel ferrocene-based substrate for the ratiometric electrochemical detection of β-galactosidase was designed and synthesised. It was demonstrated to be an excellent electrochemical substrate for β-Gal detection with sensitivity as low as 0.1 U mL-1.

β-galactosidase stability at high substrate concentrations

Enzymatic synthesis of galacto-oligosaccharides is usually performed at high initial substrate concentrations since higher yields are obtained. We report here on the stability of β-galactosidase from Bacillus circulans at 25, 40, and 60°C in buffer, and in systems with initially 5.0 and 30% (w/w) lactose. In buffer, the half-life time was 220 h and 13 h at 25 and 40°C, respectively, whereas the...